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L7759
Sigma
Lectin from Arachis hypogaea (peanut)
peroxidase conjugate, lyophilized powder
| Synonym: | Peanut agglutinin, PNA |
Description
| Biochem/physiol Actions | PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets. |
| Preparation Note | Prepared from peroxidase (P8375) using a modification of a published method1, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography. |
| Unit Definition | One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C. |
| Physical form | Contains sodium citrate |
Properties
| form | lyophilized powder |
| peroxidase activity | ≥20 units/mg protein |
| composition | Protein, ≥80% biuret |
| conjugate | peroxidase conjugate |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| Cited Reference | 1. O'Sullivan, M. J., et al., A simple method for the preparation of enzyme-antibody conjugates. FEBS Lett. 95, 311-313, (1978) Abstract |
| reference | Lotan, R. J. Biol. Chem. 250, 8518, (1975) |
| London, J. J. Immunol. 121, 438, (1978) | |
| Bird, G. W. G. Vox Sang. 9, 748, (1964) |
